2018-11-08 13:24:002025-06-13 12:21:12
description
mediator of RecA binding to ssDNA, required for the formation of RecA DNA repair centers, required for efficient survival and replication restart after replication-transcription conflicts
mediator of [[protein|RecA]] binding to ssDNA, required for the formation of [[protein|RecA]] DNA repair centers, required for efficient survival and replication restart after replication-transcription conflicts
locus
BSU25280
BSU_25280
geneLength
765
768
product
mediator of RecA binding to ssDNA
mediator of [[protein|RecA]] binding to ssDNA
outlinks
bsu
BSU25280
BSU_25280
Gene
Coordinates
2,608,946 → 2,609,713
2,608,946 2,609,713
Gene
Phenotypes of a mutant
drastically reduced survival of mature dormant spores after exposure to ultrahigh vacuum desiccation and ionizing radiation that induce single strand (ss) DNA nicks and double-strand breaks (DSBs) [Pubmed|24285298]
''[[gene|recO]] [[gene|recJ]]'' double mutants are extremely sensitive against DNA damaging agents [Pubmed|26001966]
''[[gene|recO]] [[gene|dprA]]'' double mutants have a strongly reduced chromosomal transformation rate [Pubmed|23779106,22373918]
''[[gene|recO]] [[gene|addA]]-[[gene|addB]]'' double mutants are extremely sensitive against DNA damaging agents [Pubmed|26001966]
drastically reduced survival of mature dormant spores after exposure to ultrahigh vacuum desiccation and ionizing radiation that induce single strand (ss) DNA nicks and double-strand breaks (DSBs) [Pubmed|24285298]
''[[gene|recO]] [[gene|recJ]]'' double mutants are extremely sensitive against DNA damaging agents [Pubmed|26001966]
''[[gene|recO]] [[gene|dprA]]'' double mutants have a strongly reduced chromosomal transformation rate [Pubmed|23779106,22373918]
''[[gene|recO]] [[gene|addA]]-[[gene|addB]]'' double mutants are extremely sensitive against DNA damaging agents [Pubmed|26001966]
reduced viability of a [[gene|rarA]] [[gene|recO]] double mutant [pubmed|32117122]
suppression of lethality of [[gene|pcrA]] inactivation [pubmed|32793628]
The protein
Catalyzed reaction/ biological activity
provides [[protein|RecA]] access to ssDNA during chromosomal transformation (together with [[protein|DprA]]) [Pubmed|22373918]
catalyzes annealing of [[protein|SsbA]] or [[protein|SsbA]]/[[protein|SsbB]] coated ssDNAs to allow the formation of DNA duplexes with tails during plasmid transformation [Pubmed|22373918]
required for the formation of [[protein|RecA]] DNA repair centers (together with [[protein|RecR]]) [Pubmed|24891441]
provides [[protein|RecA]] access to ssDNA during chromosomal transformation (together with [[protein|DprA]]) [Pubmed|22373918]
catalyzes annealing of [[protein|SsbA]] or [[protein|SsbA]]/[[protein|SsbB]] coated ssDNAs to allow the formation of DNA duplexes with tails during plasmid transformation [Pubmed|22373918]
required for the formation of [[protein|RecA]] DNA repair centers (together with [[protein|RecR]]) [Pubmed|24891441]
anneals [[protein|SsbA]]- or [[protein|SsbB]]-coated complementary strands of transfecting SPP1 phage DNA, yielding tailed SPP1 duplex intermediates [pubmed|31876108]
[[protein|DprA]], [[protein|RecO]] or viral single strand annealing G35P protein, may catalyze the annealing of complete linear phage genomes with redundant regions at the ends of the molecule, alone or with the help of an exonuclease, to produce a circular unit-length duplex viral genome ready to initiate replication [pubmed|31876108]
The protein
Protein family
recO family (according to Swiss-Prot)
recO family (single member, according to UniProt)
Biological materials
Mutant
MGNA-C494 (yqxN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2492 NBRP B. subtilis, Japan]
1A892 ( ''recO''::''cat''), [Pubmed|10323239], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A892&Search=1A892 BGSC]
BKE25280 ( ''recO''::''ermC''), (available at the [http://www.bgsc.org/ BGSC] and in [SW|Fabian Commichau]'s lab) [pubmed|28189581]
BP738 ( ''recO''::''tet''), (available in [SW|Fabian Commichau]'s lab)
BP774 ( ''recO''::''ermC''), (available in [SW|Fabian Commichau]'s lab)
BKE25280 (Δ[[gene|recO]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25280 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCCGCACCTTCCTCAA, downstream forward: _UP4_TGACATTTGGTCCATCTTTT
BKK25280 (Δ[[gene|recO]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25280 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCCGCACCTTCCTCAA, downstream forward: _UP4_TGACATTTGGTCCATCTTTT
GP3527 (Δ[[gene|recO]]::kan), available in [SW|Jörg Stülke]'s lab
MGNA-C494 (yqxN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2492 NBRP B. subtilis, Japan]
1A892 ( ''recO''::''cat''), [Pubmed|10323239], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A892&Search=1A892 BGSC]
BG128 (Δ[[gene|recO]]::cat), available in [SW|Juan Alonso]'s and [SW|Jörg Stülke]'s labs [pubmed|9642195]
BP738 ( Δ''recO''::''tet''), (available in [SW|Fabian Commichau]'s lab)
BP774 ( Δ''recO''::''ermC''), (available in [SW|Fabian Commichau]'s lab)
BKE25280 (Δ[[gene|recO]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25280 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCCGCACCTTCCTCAA, downstream forward: _UP4_TGACATTTGGTCCATCTTTT
BKK25280 (Δ[[gene|recO]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25280 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCCGCACCTTCCTCAA, downstream forward: _UP4_TGACATTTGGTCCATCTTTT
References
Reviews
References
Original publications
The protein
Paralogous protein(s)
[[this]]